Tetrazolium Testing.
We can test your seed viability with our Tetrazolium test to learn how many of your seeds are alive, especially if you plan to store your seeds.
The Tetrazolium Test is the classic quick test in that it provides a rapid determination of seed viability. A sample of the seed is prepared for the test by soaking in water to soften it and then cutting through impermeable membranes to permit the Tetrazolium stain to enter the seed tissue. Living tissue stains red and dead tissue does not stain. This enables us to perform a rapid test for seed viability. Viable seed may be Normal, Abnormal, Hard or Fresh in the Germination Test. Thus the Tetrazolium Test will not detect Abnormal Seedlings or dormancy.
About the Analysis
- Viability results can be available in 24 to 48 hours.
- Where dormancy is an issue with the Germination Test, the Tetrazolium Test will detect all viable seed
- The Tetrazolium Test usually requires an overnight soak in water before we cut into the embryo and transfer the seed to the tetrazolium stain. After that we allow the stain to penetrate the seed and react with live tissues before we “read” the result.
- The test is useful as a very quick check for viability. It can be carried out on fine seeds through to large grains.
Interpreting results
- Results appear as percentage (%) viable by number.
- Seed that is old, or damaged by heat, moisture and insects will have a low viability. Sometimes there is partial staining in the Tetrazolium Test - If there is enough staining including critical parts the seed is considered viable, although these seed lots usually have low vigour.
Quantity required
Quantity required:
- Normally we ask for the same quantity as is required for a Purity Test (the two tests can come from the same sample).
- Where only Tetrazolium Tests are requested we need at least 400 seed, preferably more.
Other recommended tests
Other analysis that may be carried out to augment and add value to Seed Tetrazolium Tests:
- Purity
- Germination
- Vigour
- Moisture
- Seed Count
