Tetrazolium Testing.

We can test your seed viability with our Tetrazolium test to learn how many of your seeds are alive, especially if you plan to store your seeds.

The Tetrazolium Test is the classic quick test in that it provides a rapid determination of seed viability. A sample of the seed is prepared for the test by soaking in water to soften it and then cutting through impermeable membranes to permit the Tetrazolium stain to enter the seed tissue. Living tissue stains red and dead tissue does not stain. This enables us to perform a rapid test for seed viability. Viable seed may be Normal, Abnormal, Hard or Fresh in the Germination Test. Thus the Tetrazolium Test will not detect Abnormal Seedlings or dormancy.

Tetrazolium - AgEtal

About the Analysis

  • Viability results can be available in 24 to 48 hours. 
  • Where dormancy is an issue with the Germination Test, the Tetrazolium Test will detect all viable seed
  • The Tetrazolium Test usually requires an overnight soak in water before we cut into the embryo and transfer the seed to the tetrazolium stain. After that we allow the stain to penetrate the seed and react with live tissues before we “read” the result.
  • The test is useful as a very quick check for viability. It can be carried out on fine seeds through to large grains.

Interpreting results

  • Results appear as percentage (%) viable by number.
  • Seed that is old, or damaged by heat, moisture and insects will have a low viability. Sometimes there is partial staining in the Tetrazolium Test - If there is enough staining including critical parts the seed is considered viable, although these seed lots usually have low vigour.

Quantity required

Quantity required:

  • Normally we ask for the same quantity as is required for a Purity Test (the two tests can come from the same sample).
  • Where only Tetrazolium Tests are requested we need at least 400 seed, preferably more.

Other recommended tests

Other analysis that may be carried out to augment and add value to Seed Tetrazolium Tests:

  • Purity
  • Germination
  • Vigour
  • Moisture
  • Seed Count

Tetrazolium Prices

About Tetrazolium Testing

  • Group 1 - $133.10 Wheat, Barley, Durum, Mungbean, Regur Mungbean, Cowpea, Lucern, Maize
  • Group 2 - $138.60 Atro, Japanese Millet, Pearl Millet, White French Millet, Shirohie Millet, Chickpea
  • Group 3 - $193.16 Soybean, Sorghum, Oats, Sunflower, Stylo, Purple Pigeon Grass, Setaria 
  • Group 4 - $230.78
  • Digitaria, Bluegrass, Silk Sorghum, Bambatsi, Canary Grass, Panorama
  • Group 5 - $302.83 Rhodes Grass, Signal Grass, Buffel
  • Group 6 - $320.21 Pelleted TZ

Ready to get started?  Request for Tetrazolium Testing

Would you prefer to Print or Download this form?

If you would prefer not to complete this form online, you can download or print the form.

Simply complete the form on paper, attach to your sample and post or deliver to AgEtal.

If you require testing to full ISTA methods (i.e. 4 reps instead of 2) or for OIC/BIC please use the ISTA form.

Download ISTA Form

Request for Analysis FormNew Customer Form

Accurate results
in a timely manner.


Request for Analysis.

Simply book online for all your seed and grain testing then drop off or post the sample to our lab.

Please be sure about the tests you require before submitting your Request for Analysis. *See our Cancellation Policy
Test results have a fast turnaround and are available online.

Request for your samples to be analysed in three easy steps.

Complete the
form below.
Sign & attach the receipt
to your sample.
Post or deliver the
sample to AgEtal.

Attach the form to your sample. Post or deliver the sample to AgEtal. For fast turnaround, please deliver samples before 3:30pm.

Thank you for requesting an analysis

We have received your request for analysis, don't forget to print and sign the receipt below to attach to your samples. Send or deliver your samples to our laboratory. If you have any questions, please contact us.

We will be in contact with you when your results are ready.

Print Receipt

Attach the form to your sample. Post or deliver the sample to AgEtal.

AgEtal Request Analysis

Customer Details
Accounts Payable Details
Analysis Details 1
Lab. No. (Office use only)
Analysis Details 2
Lab. No. (Office use only)
Analysis Details 3
Lab. No. (Office use only)
Analysis Details 4
Lab. No. (Office use only)
Analysis Details 5
Lab. No. (Office use only)
Analysis Details 6
Lab. No. (Office use only)
Analysis Details 7
Lab. No. (Office use only)
Analysis Details 8
Lab. No. (Office use only)
Accredited Sampler & Other

* Abbreviations: Purity = P  Germination = G  Vigour = V  Tetrazolium = Tz  |  Weed Search = WS

A booking receipt will generate when submiting this form. (a confirmation will sent to the nominated Customer email)

Test results have a fast turnaround and are available online.

Please remember to complete the final steps when you submit this form.

Sign & attach the receipt
to your sample.

(Booking Receipt)
 Post or deliver the
sample to AgEtal.

Export Information (if applicable)
Analysis Submit
Issued by Luanne Cunliffe Director Webform WAM03 01/09/21

Contact AgEtal Agricultural Testing Laboratory

Get in contact with our laboratory to book an analysis or learn more about our testing.

Phone 07 4633 3223

Courier 9/24 Carroll St Toowoomba QLD 4350

Auspost PO Box 7135 Toowoomba South 4350

Opening Hours

Monday to Friday
8:30am to 5:00pm

Saturday & Sunday
Closed to the public

Counting is done every day – after hours drop off box available.

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